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Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in parietal cells.
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in parietal cells.
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in parietal cells.

Anti-MUC3A Antibody

HPA010871
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMUC3A
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MUC3A Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4584
  • Target name
    MUC3A
  • Target description
    mucin 3A, cell surface associated
  • Target synonyms
    MUC-3A, MUC3, mucin-3A, intestinal mucin, mucin 3, intestinal
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ02505
  • Protein Name
    Mucin-3A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of MUC3A/B using anti-MUC3A/B antibody (A08332-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC3A/B antigen affinity purified polyclonal antibody (Catalog # A08332-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MUC3A/B at approximately 345 kDa. The expected band size for MUC3A/B is at 345 kDa.
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