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Anti-MUC6 Antibody (Gastric Mucin)

188-10586
RayBiotech
ApplicationsFlow Cytometry
Product group Antibodies
TargetMUC6
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-MUC6 Antibody (Gastric Mucin)
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry
  • Certification
    Research Use Only
  • Concentration
    0.2 mg/ml
  • Gene ID4588
  • Target name
    MUC6
  • Target description
    mucin 6, oligomeric mucus/gel-forming
  • Target synonyms
    gastric mucin-6; MUC-6; mucin-6
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ6W4X9
  • Protein Name
    Mucin-6
  • Scientific Description
    Mouse anti-Human MUC6 Antibody (Gastric Mucin)
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Gastric Mucin using anti-Gastric Mucin antibody (A02143). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse stomach tissue lysates, Lane 2: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gastric Mucin antigen affinity purified polyclonal antibody (Catalog # A02143) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gastric Mucin at approximately 257KD. The expected band size for Gastric Mucin is at 257KD.
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