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The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA048458(MUC6 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA048458(MUC6 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA048458(MUC6 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)

MUC6 Antibody

CSB-PA048458
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMUC6
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Overview

  • Supplier
    Cusabio
  • Product Name
    MUC6 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4588
  • Target name
    MUC6
  • Target description
    mucin 6, oligomeric mucus/gel-forming
  • Target synonyms
    gastric mucin-6; MUC-6; mucin-6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6W4X9
  • Protein Name
    Mucin-6
  • Scientific Description
    The MUC6 gene encodes gastric mucin, a secreted glycoprotein that plays an essential role in epithelial cytoprotection from acid, proteases, pathogenic microorganisms, and mechanical trauma in the gastrointestinal tract. May provide a mechanism for modulation of the composition of the protective mucus layer related to acid secretion or the presence of bacteria and noxious agents in the lumen. Plays an important role in the cytoprotection of epithelial surfaces and are used as tumor markers in a variety of cancers. May play a role in epithelial organogenesis.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Gastric Mucin using anti-Gastric Mucin antibody (A02143). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse stomach tissue lysates, Lane 2: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Gastric Mucin antigen affinity purified polyclonal antibody (Catalog # A02143) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Gastric Mucin at approximately 257KD. The expected band size for Gastric Mucin is at 257KD.
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