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Immunohistochemical staining of human colon shows moderate cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human colon shows moderate cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human colon shows moderate cytoplasmic and nuclear positivity in glandular cells.

Anti-MVD Antibody

HPA041404
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMVD
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MVD
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4597
  • Target name
    MVD
  • Target description
    mevalonate diphosphate decarboxylase
  • Target synonyms
    FP17780, MDDase, MPD, POROK7, diphosphomevalonate decarboxylase, mevalonate (diphospho) decarboxylase, mevalonate pyrophosphate decarboxylase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP53602
  • Protein Name
    Diphosphomevalonate decarboxylase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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FAQ

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Figure 1. Western blot analysis of MVD using anti-MVD antibody (A01631-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MVD antigen affinity purified polyclonal antibody (Catalog # A01631-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MVD at approximately 45KD. The expected band size for MVD is at 43KD.
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