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Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular as well as lymphoid cells.
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular as well as lymphoid cells.
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in glandular as well as lymphoid cells.

Anti-MX1 Antibody

HPA030917
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMX1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MX1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4599
  • Target name
    MX1
  • Target description
    MX dynamin like GTPase 1
  • Target synonyms
    IFI-78K, IFI78, MX, MxA, lncMX1-215, interferon-induced GTP-binding protein Mx1, interferon-induced protein p78, interferon-inducible protein p78, interferon-regulated resistance GTP-binding protein MxA, myxoma resistance protein 1, myxovirus (influenza virus) resistance 1, interferon-inducible protein p78
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20591
  • Protein Name
    Interferon-induced GTP-binding protein Mx1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Western Blot Positive WB detected in: A549 whole cell lysate, NIH/3T3 whole cell lysate, Hela whole cell lysate All lanes: MX1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76, 56 kDa Observed band size: 76 kDa
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Figure 1. Western blot analysis of MX1 using anti-MX1 antibody (A00849-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MX1 antigen affinity purified polyclonal antibody (Catalog # A00849-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MX1 at approximately 76 kDa. The expected band size for MX1 is at 76 kDa.
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Lane 1: T47D cell lysates probed with MX1 Monoclonal Antibody, Unconjugated (bsm-51528M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & cytosol.
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