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Western Blot Positive WB detected in: A549 whole cell lysate, NIH/3T3 whole cell lysate, Hela whole cell lysate All lanes: MX1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76, 56 kDa Observed band size: 76 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, NIH/3T3 whole cell lysate, Hela whole cell lysate All lanes: MX1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76, 56 kDa Observed band size: 76 kDa
Western Blot Positive WB detected in: A549 whole cell lysate, NIH/3T3 whole cell lysate, Hela whole cell lysate All lanes: MX1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 76, 56 kDa Observed band size: 76 kDa

MX1 Antibody

CSB-PA015249LA01HU
Cusabio
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetMX1
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Overview

  • Supplier
    Cusabio
  • Product Name
    MX1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4599
  • Target name
    MX1
  • Target description
    MX dynamin like GTPase 1
  • Target synonyms
    IFI78; IFI-78K; interferon-induced GTP-binding protein Mx1; interferon-induced protein p78; interferon-inducible protein p78; interferon-regulated resistance GTP-binding protein MxA; lncMX1-215; MX; MxA; myxoma resistance protein 1; myxovirus (influenza virus) resistance 1, interferon-inducible protein p78
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20591
  • Protein Name
    Interferon-induced GTP-binding protein Mx1
  • Scientific Description
    Interferon-induced dynamin-like GTPase with antiviral activity against a wide range of RNA viruses and some DNA viruses. Its target viruses include negative-stranded RNA viruses and HBV through binding and inactivation of their ribonucleocapsid. May also antagonize reoviridae and asfarviridae replication. Inhibits thogoto virus (THOV) replication by preventing the nuclear import of viral nucleocapsids. Inhibits La Crosse virus (LACV) replication by sequestering viral nucleoprotein in perinuclear complexes, preventing genome amplification, budding, and egress. Inhibits influenza A virus (IAV) replication by decreasing or delaying NP synthesis and by blocking endocytic traffic of incoming virus particles. Enhances ER stress-mediated cell death after influenza virus infection. May regulate the calcium channel activity of TRPCs.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MX1 using anti-MX1 antibody (A00849-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MX1 antigen affinity purified polyclonal antibody (Catalog # A00849-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MX1 at approximately 76 kDa. The expected band size for MX1 is at 76 kDa.
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