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Immunohistochemical staining of human skeletal muscle shows moderate to strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows moderate to strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human skeletal muscle shows moderate to strong cytoplasmic positivity in myocytes.

Anti-MYH3 Antibody

HPA021808
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMYH3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MYH3 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4621
  • Target name
    MYH3
  • Target description
    myosin heavy chain 3
  • Target synonyms
    CPSFS1A, CPSFS1B, CPSKF1A, CPSKF1B, DA2A, DA2B, DA2B3, DA8, HEMHC, MYHC-EMB, MYHSE1, SMHCE, myosin-3, myosin heavy chain, fast skeletal muscle, embryonic, myosin, heavy chain 3, skeletal muscle, embryonic, myosin, heavy polypeptide 3, skeletal muscle, embryonic, myosin, skeletal, heavy chain, embryonic 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11055
  • Protein Name
    Myosin-3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of heavy chain Myosin/MYH3 using anti-heavy chain Myosin/MYH3 antibody (A02697-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-heavy chain Myosin/MYH3 antigen affinity purified polyclonal antibody (Catalog # A02697-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for heavy chain Myosin/MYH3 at approximately 230 kDa. The expected band size for heavy chain Myosin/MYH3 is at 224 kDa.
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