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IHC-P analysis of mouse skeletal muscle tissue using GTX32147 MYH3 antibody. Working concentration : 5 microg/ml
IHC-P analysis of mouse skeletal muscle tissue using GTX32147 MYH3 antibody. Working concentration : 5 microg/ml
IHC-P analysis of mouse skeletal muscle tissue using GTX32147 MYH3 antibody. Working concentration : 5 microg/ml

MYH3 antibody

GTX32147
GeneTex
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetMYH3
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Overview

  • Supplier
    GeneTex
  • Product Name
    MYH3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 microg/mL. IHC-P: 5 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4621
  • Target name
    MYH3
  • Target description
    myosin heavy chain 3
  • Target synonyms
    CPSFS1A, CPSFS1B, CPSKF1A, CPSKF1B, DA2A, DA2B, DA2B3, DA8, HEMHC, MYHC-EMB, MYHSE1, SMHCE, myosin-3, myosin heavy chain, fast skeletal muscle, embryonic, myosin, heavy chain 3, skeletal muscle, embryonic, myosin, heavy polypeptide 3, skeletal muscle, embryonic, myosin, skeletal, heavy chain, embryonic 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP11055
  • Protein Name
    Myosin-3
  • Scientific Description
    Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. This gene is a member of the MYH family and encodes a protein with an IQ domain and a myosin head-like domain. Mutations in this gene have been associated with two congenital contracture (arthrogryposis) syndromes, Freeman-Sheldon syndrome and Sheldon-Hall syndrome. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

References

  • Vaiman A, Fritz S, Beauvallet C, et al. Mutation of the MYH3 gene causes recessive cleft palate in Limousine cattle. Genet Sel Evol. 2022,54(1):71. doi: 10.1186/s12711-022-00762-2
    Read this paper
  • Zieba J, Zhang W, Chong JX, et al. A postnatal role for embryonic myosin revealed by MYH3 mutations that alter TGFβ signaling and cause autosomal dominant spondylocarpotarsal synostosis. Sci Rep. 2017,7:41803. doi: 10.1038/srep41803
    Read this paper

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Figure 1. Western blot analysis of heavy chain Myosin/MYH3 using anti-heavy chain Myosin/MYH3 antibody (A02697-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-heavy chain Myosin/MYH3 antigen affinity purified polyclonal antibody (Catalog # A02697-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for heavy chain Myosin/MYH3 at approximately 230 kDa. The expected band size for heavy chain Myosin/MYH3 is at 224 kDa.
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