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Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.

Anti-MYL3 Antibody

HPA063034
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMYL3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MYL3
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4634
  • Target name
    MYL3
  • Target description
    myosin light chain 3
  • Target synonyms
    CMH8, MLC-lV/sb, MLC1SB, MLC1V, VLC1, VLCl, myosin light chain 3, CMLC1, cardiac myosin light chain 1, myosin, light chain 3, alkali; ventricular, skeletal, slow, myosin, light polypeptide 3, alkali; ventricular, skeletal, slow, ventricular myosin alkali light chain, ventricular myosin light chain 1, ventricular/slow twitch myosin alkali light chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08590
  • Protein Name
    Myosin light chain 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of MYL3 using anti-MYL3 antibody (A04730-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human H9C2(2-1) whole cell lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse skeletal muscle tissue lysates, Lane 6: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYL3 antigen affinity purified polyclonal antibody (Catalog # A04730-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYL3 at approximately 25 kDa. The expected band size for MYL3 is at 22 kDa.
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