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Western Blot analysis of Jurkat cells using MYL3 Polyclonal Antibody
Western Blot analysis of Jurkat cells using MYL3 Polyclonal Antibody
Western Blot analysis of Jurkat cells using MYL3 Polyclonal Antibody

MYL3 Antibody

CSB-PA003345
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetMYL3
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Overview

  • Supplier
    Cusabio
  • Product Name
    MYL3 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4634
  • Target name
    MYL3
  • Target description
    myosin light chain 3
  • Target synonyms
    cardiac myosin light chain 1; CMH8; CMLC1; MLC1SB; MLC1V; MLC-lV/sb; myosin light chain 3; myosin, light chain 3, alkali; ventricular, skeletal, slow; myosin, light polypeptide 3, alkali; ventricular, skeletal, slow; ventricular myosin alkali light chain; ventricular myosin light chain 1; ventricular/slow twitch myosin alkali light chain; VLC1; VLCl
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08590
  • Protein Name
    Myosin light chain 3
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MYL3 using anti-MYL3 antibody (A04730-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Caco-2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human H9C2(2-1) whole cell lysates, Lane 4: mouse heart tissue lysates, Lane 5: mouse skeletal muscle tissue lysates, Lane 6: mouse C2C12 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYL3 antigen affinity purified polyclonal antibody (Catalog # A04730-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYL3 at approximately 25 kDa. The expected band size for MYL3 is at 22 kDa.
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