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Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human smooth muscle shows cytoplasmic positivity in smooth muscle cells.

Anti-MYL6 Antibody

HPA046859
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetMYL6
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-MYL6
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4637
  • Target name
    MYL6
  • Target description
    myosin light chain 6
  • Target synonyms
    17 kDa myosin light chain; ESMLC; LC17; LC17A; LC17B; LC17-GI; LC17-NM; MLC1SM; MLC-3; MLC3NM; MLC3SM; myosin light chain A3; myosin light chain alkali 3; myosin light polypeptide 6; myosin, light chain 6, alkali, smooth muscle and non-muscle; myosin, light polypeptide 6, alkali, smooth muscle and non-muscle
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP60660
  • Protein Name
    Myosin light polypeptide 6
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MYL6 using anti-MYL6 antibody (A09646-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYL6 antigen affinity purified polyclonal antibody (Catalog # A09646-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYL6 at approximately 16 kDa. The expected band size for MYL6 is at 16 kDa.
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