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MYL6 Antibody

LS-C410092
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetMYL6
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    MYL6 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 16kDa, while the observed MW by Western blot was 14kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 16kDa, while the observed MW by Western blot was 14kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4637
  • Target name
    MYL6
  • Target description
    myosin light chain 6
  • Target synonyms
    ESMLC, LC17, LC17-GI, LC17-NM, LC17A, LC17B, MLC-3, MLC1SM, MLC3NM, MLC3SM, myosin light polypeptide 6, 17 kDa myosin light chain, myosin light chain A3, myosin light chain alkali 3, myosin, light chain 6, alkali, smooth muscle and non-muscle, myosin, light polypeptide 6, alkali, smooth muscle and non-muscle
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MYL6 using anti-MYL6 antibody (A09646-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human THP-1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYL6 antigen affinity purified polyclonal antibody (Catalog # A09646-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYL6 at approximately 16 kDa. The expected band size for MYL6 is at 16 kDa.
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