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Anti-MYLK3 Antibody

144-66204
RayBiotech
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
ReactivityHuman, Mouse
TargetMYLK3
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-MYLK3 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID91807
  • Target name
    MYLK3
  • Target description
    myosin light chain kinase 3
  • Target synonyms
    MLCK, MLCK2, caMLCK, myosin light chain kinase 3, MLC kinase, cardiac-MLCK, cardiac-MyBP-C associated Ca/CaM kinase, putative myosin light chain kinase 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ32MK0
  • Protein Name
    Myosin light chain kinase 3
  • Scientific Description
    MYLK3 pAb
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of MYLK3 using anti-MYLK3 antibody (A09180-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYLK3 antigen affinity purified polyclonal antibody (Catalog # A09180-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYLK3 at approximately 100 kDa. The expected band size for MYLK3 is at 88 kDa.
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