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Mouse bone lysates probed with MYLK3 Polyclonal Antibody, Unconjugated (bs-9865R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Mouse bone lysates probed with MYLK3 Polyclonal Antibody, Unconjugated (bs-9865R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Mouse bone lysates probed with MYLK3 Polyclonal Antibody, Unconjugated (bs-9865R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

MYLK3 Polyclonal Antibody

BS-9865R
Bioss
ApplicationsWestern Blot
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Rabbit, Rat, Sheep
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Overview

  • Supplier
    Bioss
  • Product Name
    MYLK3 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(1:300-5000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Bovine, Canine, Equine, Human, Mouse, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Hu S, Cheng M, Guo X, et al. Down-regulation of miR-200c attenuates AngII-induced cardiac hypertrophy via targeting the MLCK-mediated pathway. J Cell Mol Med. 2019,23(4):2505-2516. doi: 10.1111/jcmm.14135
    Read this paper

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Figure 1. Western blot analysis of MYLK3 using anti-MYLK3 antibody (A09180-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEL whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human Caco-2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYLK3 antigen affinity purified polyclonal antibody (Catalog # A09180-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYLK3 at approximately 100 kDa. The expected band size for MYLK3 is at 88 kDa.
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