Human Myosin IIA (non-muscle) (heavy chain) is detected by immunocytochemistry using anti-myosin IIA (non-muscle) (rec.) (SF9) (Prod. No. AG-27B-0010). Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incu
anti-Myosin IIA (non-muscle) (heavy chain), mAb (rec.) (SF9)
AG-27B-0010
ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityDrosophila, Human, Mouse, Rat
TargetMYH9
Overview
- SupplierAdipoGen Life Sciences
- Product Nameanti-Myosin IIA (non-muscle) (heavy chain), mAb (rec.) (SF9)
- Delivery Days Customer10
- Antibody SpecificityRecognizes human, mouse, rat and drosophila myosin IIA (heavy chain).
- ApplicationsWestern Blot, ELISA, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDSF9
- Concentration1 mg/ml
- Estimated Purity>95%
- FormulationLiquid
- Gene ID4627
- Target nameMYH9
- Target descriptionmyosin heavy chain 9
- Target synonymsBDPLT6; cellular myosin heavy chain, type A; DFNA17; EPSTS; FTNS; MATINS; MHA; myosin, heavy chain 9, non-muscle; myosin-9; NMHC-II-A; NMMHCA; NMMHC-IIA; non-muscle myosin heavy chain 9; non-muscle myosin heavy chain A; non-muscle myosin heavy chain IIa; nonmuscle myosin heavy chain II-A; non-muscle myosin heavy polypeptide 9
- HostHuman
- IsotypeIgG2
- Protein IDP35579
- Protein NameMyosin-9
- Scientific Descriptionanti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein. - Recombinant Antibody. Recognizes human, mouse, rat and drosophila myosin IIA (heavy chain). Species cross-reactivity: Drosophila, Human, Mouse, Rat. Clone: SF9. Isotype: Human IgG2lambda. Applications: ELISA, ICC, WB. Host: Purified from HEK 293 cell culture supernatant. Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. anti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
- ReactivityDrosophila, Human, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203