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Immunohistochemical staining of human hippocampus shows strong nuclear membranous positivity in neuronal cells.
Immunohistochemical staining of human hippocampus shows strong nuclear membranous positivity in neuronal cells.
Immunohistochemical staining of human hippocampus shows strong nuclear membranous positivity in neuronal cells.

Anti-NANP Antibody

HPA050342
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetNANP
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NANP
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID140838
  • Target name
    NANP
  • Target description
    N-acetylneuraminic acid phosphatase
  • Target synonyms
    C20orf147; dJ694B14.3; haloacid dehalogenase-like hydrolase domain containing 4; haloacid dehalogenase-like hydrolase domain-containing protein 4; HDHD4; N-acylneuraminate-9-phosphatase; Neu5Ac-9-Pase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TBE9
  • Protein Name
    N-acylneuraminate-9-phosphatase
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NANP using anti-NANP antibody (A09658). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NANP antigen affinity purified polyclonal antibody (Catalog # A09658) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NANP at approximately 30 kDa. The expected band size for NANP is at 28,30 kDa.
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