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ProductsBack/NANP antibody, Internal
WB analysis of Jurkat cells using GTX45629 NANP antibody at 0.2-1microg/ml.
WB analysis of Jurkat cells using GTX45629 NANP antibody at 0.2-1microg/ml.
WB analysis of Jurkat cells using GTX45629 NANP antibody at 0.2-1microg/ml.

NANP antibody, Internal

GTX45629
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetNANP
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Overview

  • Supplier
    GeneTex
  • Product Name
    NANP antibody, Internal
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID140838
  • Target name
    NANP
  • Target description
    N-acetylneuraminic acid phosphatase
  • Target synonyms
    C20orf147, HDHD4, dJ694B14.3, N-acylneuraminate-9-phosphatase, N-acetylneuraminate-9-phosphate phosphatase, Neu5Ac-9-Pase, haloacid dehalogenase-like hydrolase domain containing 4, haloacid dehalogenase-like hydrolase domain-containing protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8TBE9
  • Protein Name
    N-acylneuraminate-9-phosphatase
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NANP using anti-NANP antibody (A09658). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NANP antigen affinity purified polyclonal antibody (Catalog # A09658) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NANP at approximately 30 kDa. The expected band size for NANP is at 28,30 kDa.
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