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Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in Paneth cells.
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in Paneth cells.
Immunohistochemical staining of human small intestine shows strong cytoplasmic positivity in Paneth cells.

Anti-NCF1 Antibody

HPA052095
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetNCF1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NCF1
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID653361
  • Target name
    NCF1
  • Target description
    neutrophil cytosolic factor 1
  • Target synonyms
    47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; CGD1; NADPH oxidase organizer 2; NCF-1; NCF1A; NCF-47K; neutrophil cytosol factor 1; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1); neutrophil NADPH oxidase factor 1; nox organizer 2; NOXO2; nox-organizing protein 2; p47phox; p47-phox; SH3 and PX domain-containing protein 1A; SH3PXD1A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14598
  • Protein Name
    Neutrophil cytosol factor 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NCF1 using anti-NCF1 antibody (A01586-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human blood lysates, Lane 3: rat thymus tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCF1 antigen affinity purified polyclonal antibody (Catalog # A01586-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCF1 at approximately 47KD. The expected band size for NCF1 is at 44KD.
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