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Anti-NCKAP1 Antibody

144-12229
RayBiotech
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNCKAP1
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-NCKAP1 Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10787
  • Target name
    NCKAP1
  • Target description
    NCK associated protein 1
  • Target synonyms
    HEM2, NAP1, NAP125, p125Nap1, nck-associated protein 1, membrane-associated protein HEM-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y2A7
  • Protein Name
    Nck-associated protein 1
  • Scientific Description
    NCKAP1 Polyclonal Antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NCKAP1 using anti-NCKAP1 antibody (A09238-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: MCF-7 whole Cell lysates, Lane 4: 293T whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCKAP1 antigen affinity purified polyclonal antibody (Catalog # A09238-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCKAP1 at approximately 129KD. The expected band size for NCKAP1 is at 129KD.
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