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Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.

Anti-NCOA4 Antibody

HPA065208
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNCOA4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NCOA4
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8031
  • Target name
    NCOA4
  • Target description
    nuclear receptor coactivator 4
  • Target synonyms
    ARA70, ELE1, PTC3, RFG, nuclear receptor coactivator 4, 70 kDa AR-activator, 70 kDa androgen receptor coactivator, NCoA-4, RET-activating gene ELE1, androgen receptor-associated protein of 70 kDa, ferritin cargo receptor NCOA4, ret fused
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13772
  • Protein Name
    Nuclear receptor coactivator 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of NCOA4 using anti-NCOA4 antibody (A04368-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human Daudi whole cell lysates, Lane 7: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCOA4 antigen affinity purified polyclonal antibody (Catalog # A04368-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NCOA4 at approximately 70 kDa. The expected band size for NCOA4 is at 70 kDa.
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