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Immunohistochemical staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons.
Immunohistochemical staining of human cerebral cortex shows strong granular cytoplasmic positivity in neurons.

Anti-NDFIP1 Antibody

HPA009682
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNDFIP1
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NDFIP1 Antibody
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80762
  • Target name
    NDFIP1
  • Target description
    Nedd4 family interacting protein 1
  • Target synonyms
    N4WBP5, NEDD4 family-interacting protein 1, Nedd4 WW domain-binding protein 5, breast cancer-associated protein SGA-1M, putative MAPK-activating protein PM13, putative NF-kappa-B-activating protein 164, putative NFKB and MAPK-activating protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BT67
  • Protein Name
    NEDD4 family-interacting protein 1
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of NDFIP1 using anti-NDFIP1 antibody (A04644-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human HEL whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat C6 whole cell lysates, Lane 9: mouse brain tissue lysates, Lane 10: mouse lung tissue lysates, Lane 11: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDFIP1 antigen affinity purified polyclonal antibody (Catalog # A04644-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDFIP1 at approximately 28 kDa. The expected band size for NDFIP1 is at 25 kDa.
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