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ProductsBack/NDFIP1 / N4WBP5 Antibody

NDFIP1 / N4WBP5 Antibody

LS-C761038
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetNDFIP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NDFIP1 / N4WBP5 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:1000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID80762
  • Target name
    NDFIP1
  • Target description
    Nedd4 family interacting protein 1
  • Target synonyms
    breast cancer-associated protein SGA-1M; N4WBP5; NEDD4 family-interacting protein 1; Nedd4 WW domain-binding protein 5; putative MAPK-activating protein PM13; putative NF-kappa-B-activating protein 164; putative NFKB and MAPK-activating protein
  • Host
    Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NDFIP1 using anti-NDFIP1 antibody (A04644-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human HEL whole cell lysates, Lane 6: human SH-SY5Y whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat C6 whole cell lysates, Lane 9: mouse brain tissue lysates, Lane 10: mouse lung tissue lysates, Lane 11: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDFIP1 antigen affinity purified polyclonal antibody (Catalog # A04644-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDFIP1 at approximately 28 kDa. The expected band size for NDFIP1 is at 25 kDa.
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