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Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in interlobular ducts.
Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in interlobular ducts.
Immunohistochemical staining of human pancreas shows moderate cytoplasmic positivity in interlobular ducts.

Anti-NDST4 Antibody

HPA011129
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNDST4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NDST4
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64579
  • Target name
    NDST4
  • Target description
    N-deacetylase and N-sulfotransferase 4
  • Target synonyms
    N-HSST, N-HSST 4, NDST-4, NHSST4, bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4, N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 4, N-deacetylase/N-sulfotransferase 4, N-heparan sulfate sulfotransferase 4, glucosaminyl N-deacetylase/N-sulfotransferase 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H3R1
  • Protein Name
    Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of NDST4 using anti-NDST4 antibody (A11749-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDST4 antigen affinity purified polyclonal antibody (Catalog # A11749-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDST4 at approximately 101 kDa. The expected band size for NDST4 is at 101 kDa.
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