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ProductsBack/NDST4 Antibody (aa185-212)

NDST4 Antibody (aa185-212)

LS-C158372
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetNDST4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NDST4 Antibody (aa185-212)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID64579
  • Target name
    NDST4
  • Target description
    N-deacetylase and N-sulfotransferase 4
  • Target synonyms
    N-HSST, N-HSST 4, NDST-4, NHSST4, bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4, N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 4, N-deacetylase/N-sulfotransferase 4, N-heparan sulfate sulfotransferase 4, glucosaminyl N-deacetylase/N-sulfotransferase 4
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NDST4 using anti-NDST4 antibody (A11749-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDST4 antigen affinity purified polyclonal antibody (Catalog # A11749-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDST4 at approximately 101 kDa. The expected band size for NDST4 is at 101 kDa.
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