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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells.

Anti-NDUFA2 Antibody

HPA035933
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetNDUFA2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NDUFA2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4695
  • Target name
    NDUFA2
  • Target description
    NADH:ubiquinone oxidoreductase subunit A2
  • Target synonyms
    B8; CD14; CIB8; complex I B8 subunit; MC1DN13; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2, 8kDa; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2; NADH-ubiquinone oxidoreductase subunit CI-B8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43678
  • Protein Name
    NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NDUFA2 using anti-NDUFA2 antibody (A10396-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA2 antigen affinity purified polyclonal antibody (Catalog # A10396-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA2 at approximately 14 kDa. The expected band size for NDUFA2 is at 11 kDa.
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