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WB analysis of various sample lysates using GTX32741 NDUFA2 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32741 NDUFA2 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX32741 NDUFA2 antibody. Dilution : 1:1000 Loading : 25microg per lane

NDUFA2 antibody

GTX32741
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetNDUFA2
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Overview

  • Supplier
    GeneTex
  • Product Name
    NDUFA2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:200. IHC-P: 1:50 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4695
  • Target name
    NDUFA2
  • Target description
    NADH:ubiquinone oxidoreductase subunit A2
  • Target synonyms
    B8; CD14; CIB8; complex I B8 subunit; MC1DN13; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2, 8kDa; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2; NADH-ubiquinone oxidoreductase subunit CI-B8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO43678
  • Protein Name
    NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2
  • Scientific Description
    The encoded protein is a subunit of the hydrophobic protein fraction of the NADH:ubiquinone oxidoreductase (complex 1), the first enzyme complex in the electron transport chain located in the inner mitochondrial membrane, and may be involved in regulating complex I activity or its assembly via assistance in redox processes. Mutations in this gene are associated with Leigh syndrome, an early-onset progressive neurodegenerative disorder. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NDUFA2 using anti-NDUFA2 antibody (A10396-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA2 antigen affinity purified polyclonal antibody (Catalog # A10396-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA2 at approximately 14 kDa. The expected band size for NDUFA2 is at 11 kDa.
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