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Immunohistochemical staining of human stomach, upper shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach, upper shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach, upper shows strong granular cytoplasmic positivity in glandular cells.

Anti-NDUFA3 Antibody

HPA046976
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNDUFA3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NDUFA3
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4696
  • Target name
    NDUFA3
  • Target description
    NADH:ubiquinone oxidoreductase subunit A3
  • Target synonyms
    B9, CI-B9, NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3, NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 3, 9kDa, NADH-ubiquinone oxidoreductase B9 subunit, complex I B9 subunit, complex I-B9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95167
  • Protein Name
    NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of NDUFA3 using anti-NDUFA3 antibody (A11694-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA3 antigen affinity purified polyclonal antibody (Catalog # A11694-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA3 at approximately 9 kDa. The expected band size for NDUFA3 is at 9 kDa.
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