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IHC-P analysis of formalin fixed human breast cancer tissue section using GTX56108 NDUFA3 antibody. Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)
IHC-P analysis of formalin fixed human breast cancer tissue section using GTX56108 NDUFA3 antibody. Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)
IHC-P analysis of formalin fixed human breast cancer tissue section using GTX56108 NDUFA3 antibody. Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

NDUFA3 antibody

GTX56108
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetNDUFA3
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Overview

  • Supplier
    GeneTex
  • Product Name
    NDUFA3 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:1000. IHC-P: 1:100 - 1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4696
  • Target name
    NDUFA3
  • Target description
    NADH:ubiquinone oxidoreductase subunit A3
  • Target synonyms
    B9; CI-B9; complex I B9 subunit; complex I-B9; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 3, 9kDa; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3; NADH-ubiquinone oxidoreductase B9 subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95167
  • Protein Name
    NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NDUFA3 using anti-NDUFA3 antibody (A11694-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFA3 antigen affinity purified polyclonal antibody (Catalog # A11694-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFA3 at approximately 9 kDa. The expected band size for NDUFA3 is at 9 kDa.
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