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Immunofluorescent staining of human cell line U2OS shows localization to cytosol & mitochondria.
Immunofluorescent staining of human cell line U2OS shows localization to cytosol & mitochondria.
Immunofluorescent staining of human cell line U2OS shows localization to cytosol & mitochondria.

Anti-NDUFAF4 Antibody

HPA030216
Atlas Antibodies
ApplicationsImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetNDUFAF4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NDUFAF4
  • Delivery Days Customer
    12
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29078
  • Target name
    NDUFAF4
  • Target description
    NADH:ubiquinone oxidoreductase complex assembly factor 4
  • Target synonyms
    bA22L21.1; C6orf66; hormone-regulated proliferation-associated protein of 20 kDa; hormone-regulated proliferation-associated protein, 20 kDa; HRPAP20; HSPC125; MC1DN15; My013; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 4; NADH dehydrogenase (ubiquinone) complex I, assembly factor 4; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P032
  • Protein Name
    NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NDUFAF4 using anti-NDUFAF4 antibody (A11650-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Ramos whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFAF4 antigen affinity purified polyclonal antibody (Catalog # A11650-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFAF4 at approximately 21 kDa. The expected band size for NDUFAF4 is at 20,21,25 kDa.
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