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The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA211169(NDUFAF4 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA211169(NDUFAF4 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA211169(NDUFAF4 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: x200)

NDUFAF4 Antibody

CSB-PA211169
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNDUFAF4
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Overview

  • Supplier
    Cusabio
  • Product Name
    NDUFAF4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29078
  • Target name
    NDUFAF4
  • Target description
    NADH:ubiquinone oxidoreductase complex assembly factor 4
  • Target synonyms
    bA22L21.1; C6orf66; hormone-regulated proliferation-associated protein of 20 kDa; hormone-regulated proliferation-associated protein, 20 kDa; HRPAP20; HSPC125; MC1DN15; My013; NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 4; NADH dehydrogenase (ubiquinone) complex I, assembly factor 4; NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9P032
  • Protein Name
    NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4
  • Scientific Description
    NADH:ubiquinone oxidoreductase (complex I) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane. This gene encodes a complex I assembly factor. Mutations in this gene are a cause of mitochondrial complex I deficiency.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NDUFAF4 using anti-NDUFAF4 antibody (A11650-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Ramos whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFAF4 antigen affinity purified polyclonal antibody (Catalog # A11650-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFAF4 at approximately 21 kDa. The expected band size for NDUFAF4 is at 20,21,25 kDa.
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