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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.

Anti-NDUFB4 Antibody

HPA064441
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetNDUFB4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NDUFB4
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4710
  • Target name
    NDUFB4
  • Target description
    NADH:ubiquinone oxidoreductase subunit B4
  • Target synonyms
    B15; CI-B15; complex I B15 subunit; complex I-B15; NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 4, 15kDa; NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4; NADH-ubiquinone oxidoreductase B15 subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95168
  • Protein Name
    NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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L929 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NDUFB4) polyclonal Antibody, Unconjugated (bs-55145R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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Figure 1. Western blot analysis of NDUFB4 using anti-NDUFB4 antibody (A11016-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFB4 antigen affinity purified polyclonal antibody (Catalog # A11016-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFB4 at approximately 15 kDa. The expected band size for NDUFB4 is at 15 kDa.
Product group Antibodies
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TargetNDUFB4
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Immunohistochemistry analysis in human heart muscle and pancreas tissues using Anti-NDUFB4 antibody. Corresponding NDUFB4 RNA-seq data are presented for the same tissues.
Product group Antibodies
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Immunohistochemistry analysis in human heart muscle and pancreas tissues using Anti-NDUFB4 antibody. Corresponding NDUFB4 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-NDUFB4 AntibodyHPA051739
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetNDUFB4
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