
Figure 1. Western blot analysis of NDUFV2 using anti-NDUFV2 antibody (A05801-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: rat heart tissue lysates, Lane 7: rat brain tissue lysates, Lane 8: rat kidney tissue lysates, Lane 9: rat C6 whole cell lysates, Lane 10: mouse heart tissue lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse kidney tissue lysates, Lane 13: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFV2 antigen affinity purified polyclonal antibody (Catalog # A05801-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFV2 at approximately 27 kDa. The expected band size for NDUFV2 is at 27 kDa.
Anti-NDUFV2 Antibody Picoband(r)
A05801-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNDUFV2
Overview
- SupplierBoster Bio
- Product NameAnti-NDUFV2 Antibody Picoband(r)
- Delivery Days Customer9
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration500 ug/ml
- Gene ID4729
- Target nameNDUFV2
- Target descriptionNADH:ubiquinone oxidoreductase core subunit V2
- Target synonymsCI-24k; complex I 24kDa subunit; complex I, mitochondrial respitory chain, 24 kD subunit; MC1DN7; NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa; NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; NADH dehydrogenase ubiquinone flavoprotein 2, mitochondrial; NADH-ubiquinone oxidoreductase 24 kDa subunit; NADH-ubiquinone oxidoreductase flavoprotein 2; nuclear-encoded mitochondrial NADH-ubiquinone reductase 24Kd subunit
- HostRabbit
- IsotypeIgG
- Protein IDP19404
- Protein NameNADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial
- Scientific DescriptionBoster Bio Anti-NDUFV2 Antibody Picoband® catalog # A05801-2. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203