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ProductsBack/NDUFV2 Polyclonal Antibody
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NDUFV2) polyclonal Antibody, Unconjugated (bs-3960R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NDUFV2) polyclonal Antibody, Unconjugated (bs-3960R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
A549 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (NDUFV2) polyclonal Antibody, Unconjugated (bs-3960R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.

NDUFV2 Polyclonal Antibody

BS-3960R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNDUFV2
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    NDUFV2 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4729
  • Target name
    NDUFV2
  • Target description
    NADH:ubiquinone oxidoreductase core subunit V2
  • Target synonyms
    CI-24k; complex I 24kDa subunit; complex I, mitochondrial respitory chain, 24 kD subunit; MC1DN7; NADH dehydrogenase (ubiquinone) flavoprotein 2, 24kDa; NADH dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial; NADH dehydrogenase ubiquinone flavoprotein 2, mitochondrial; NADH-ubiquinone oxidoreductase 24 kDa subunit; NADH-ubiquinone oxidoreductase flavoprotein 2; nuclear-encoded mitochondrial NADH-ubiquinone reductase 24Kd subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of NDUFV2 using anti-NDUFV2 antibody (A05801-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: human CACO-2 whole cell lysates, Lane 6: rat heart tissue lysates, Lane 7: rat brain tissue lysates, Lane 8: rat kidney tissue lysates, Lane 9: rat C6 whole cell lysates, Lane 10: mouse heart tissue lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse kidney tissue lysates, Lane 13: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NDUFV2 antigen affinity purified polyclonal antibody (Catalog # A05801-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NDUFV2 at approximately 27 kDa. The expected band size for NDUFV2 is at 27 kDa.
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