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Immunohistochemistry analysis in human cerebral cortex and skin tissues using HPA013998 antibody. Corresponding NECAB2 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human cerebral cortex and skin tissues using HPA013998 antibody. Corresponding NECAB2 RNA-seq data are presented for the same tissues.
Immunohistochemistry analysis in human cerebral cortex and skin tissues using HPA013998 antibody. Corresponding NECAB2 RNA-seq data are presented for the same tissues.

Anti-NECAB2 Antibody

HPA013998
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetNECAB2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NECAB2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54550
  • Target name
    NECAB2
  • Target description
    N-terminal EF-hand calcium binding protein 2
  • Target synonyms
    EFCBP2, stip-2, N-terminal EF-hand calcium-binding protein 2, EF-hand calcium-binding protein 2, neuronal calcium binding 2, neuronal calcium-binding protein 2, synaptotagmin-interacting protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7Z6G3
  • Protein Name
    N-terminal EF-hand calcium-binding protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of NECAB2 using anti-NECAB2 antibody (A13886-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NECAB2 antigen affinity purified polyclonal antibody (Catalog # A13886-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NECAB2 at approximately 43 kDa. The expected band size for NECAB2 is at 43 kDa.
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