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Immunohistochemical staining of human testis shows strong nuclear positivity in subsets of cells in ductus seminiferus.
Immunohistochemical staining of human testis shows strong nuclear positivity in subsets of cells in ductus seminiferus.
Immunohistochemical staining of human testis shows strong nuclear positivity in subsets of cells in ductus seminiferus.

Anti-NEK11 Antibody

HPA016908
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
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Product group Antibodies
TargetNEK11
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NEK11
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79858
  • Target name
    NEK11
  • Target description
    NIMA related kinase 11
  • Target synonyms
    NIMA (never in mitosis gene a)- related kinase 11; serine/threonine-protein kinase Nek11
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NG66
  • Protein Name
    Serine/threonine-protein kinase Nek11
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Paraformaldehyde-fixed, paraffin embedded mouse testis; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with _x000D_ NEK11 Polyclonal Antibody, Unconjugated (bs-19195R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody for 20 minutes and DAB staining.
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Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: A549 cells, 231 cells, Primary antibody: CSB-PA299370(NEK11 Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
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Figure 1. Western blot analysis of NEK11 using anti-NEK11 antibody (A10929-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEK11 antigen affinity purified polyclonal antibody (Catalog # A10929-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEK11 at approximately 74 kDa. The expected band size for NEK11 is at 74 kDa.
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