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Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: A549 cells, 231 cells, Primary antibody: CSB-PA299370(NEK11 Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: A549 cells, 231 cells, Primary antibody: CSB-PA299370(NEK11 Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute
Gel: 10%SDS-PAGE, Lysate: 40 ug, Lane 1-2: A549 cells, 231 cells, Primary antibody: CSB-PA299370(NEK11 Antibody) at dilution 1/1000, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute

NEK11 Antibody

CSB-PA299370
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetNEK11
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Overview

  • Supplier
    Cusabio
  • Product Name
    NEK11 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79858
  • Target name
    NEK11
  • Target description
    NIMA related kinase 11
  • Target synonyms
    NIMA (never in mitosis gene a)- related kinase 11; serine/threonine-protein kinase Nek11
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NG66
  • Protein Name
    Serine/threonine-protein kinase Nek11
  • Scientific Description
    This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NEK11 using anti-NEK11 antibody (A10929-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NEK11 antigen affinity purified polyclonal antibody (Catalog # A10929-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NEK11 at approximately 74 kDa. The expected band size for NEK11 is at 74 kDa.
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