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Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts and leydig cells.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts and leydig cells.
Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts and leydig cells.

Anti-NELFA Antibody

HPA039858
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetNELFA
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NELFA
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7469
  • Target name
    NELFA
  • Target description
    negative elongation factor complex member A
  • Target synonyms
    negative elongation factor A; NELF-A; P/OKcl.15; WHSC2; wolf-Hirschhorn syndrome candidate 2 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H3P2
  • Protein Name
    Negative elongation factor A
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NELFA using anti-NELFA antibody (A07040-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NELFA antigen affinity purified polyclonal antibody (Catalog # A07040-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NELFA at approximately 57 kDa. The expected band size for NELFA is at 57 kDa.
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