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Anti-NFIC Antibody

Anti-NFIC Antibody

A40179
Antibodies.com
ApplicationsWestern Blot, ImmunoCytoChemistry
Product group Antibodies
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-NFIC Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Host
    Mouse
  • Isotype
    IgG2b
  • Scientific Description
    Mouse monoclonal antibody to NFIC
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NFIC using anti-NFIC antibody (A04154-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human Caco-2 whole cell lysates, Lane 8: rat skeletal muscle tissue lysates, Lane 9: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFIC antigen affinity purified polyclonal antibody (Catalog # A04154-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for NFIC at approximately 55-65, 70KD. The expected band size for NFIC is at 56KD.
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