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Immunohistochemical analysis of paraffin-embedded human breast cancer, using NFIC(GTX114583) antibody at 1:250 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded human breast cancer, using NFIC(GTX114583) antibody at 1:250 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
Immunohistochemical analysis of paraffin-embedded human breast cancer, using NFIC(GTX114583) antibody at 1:250 dilution. Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min

NFIC antibody

GTX114583
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetNFIC
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Overview

  • Supplier
    GeneTex
  • Product Name
    NFIC antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4782
  • Target name
    NFIC
  • Target description
    nuclear factor I C
  • Target synonyms
    CTF, CTF5, NF-I, NFI, nuclear factor 1 C-type, CCAAT-box-binding transcription factor, NF-I/C, NF1-C, TGGCA-binding protein, nuclear factor I/C (CCAAT-binding transcription factor)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08651
  • Protein Name
    Nuclear factor 1 C-type
  • Scientific Description
    Recognizes and binds the palindromic sequence 5-TTGGCNNNNNGCCAA-3 present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NFIC using anti-NFIC antibody (A04154-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human T-47D whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human Caco-2 whole cell lysates, Lane 8: rat skeletal muscle tissue lysates, Lane 9: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFIC antigen affinity purified polyclonal antibody (Catalog # A04154-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for NFIC at approximately 55-65, 70KD. The expected band size for NFIC is at 56KD.
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