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Anti-NFKB2 [SAIC-26C-15]

Ab00318-1.1
Absolute Antibody
ApplicationsMass Spectrometry
Product group Antibodies
TargetNFKB2
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-NFKB2 [SAIC-26C-15]
  • Delivery Days Customer
    7
  • Application Supplier Note
    Original data characterizing this antibody may be found here .
  • Applications
    Mass Spectrometry
  • Applications Supplier
    immunoMRM
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SAIC-26C-15
  • Gene ID4791
  • Target name
    NFKB2
  • Target description
    nuclear factor kappa B subunit 2
  • Target synonyms
    CVID10; DNA-binding factor KBF2; H2TF1; lymphocyte translocation chromosome 10 protein; LYT10; LYT-10; NFKB, p52/p100 subunit; NF-kB2; nuclear factor Kappa-B, subunit 2; nuclear factor NF-kappa-B p100 subunit; nuclear factor NF-kappa-B p52 subunit; nuclear factor of Kappa light chain gene enhancer in B cells 2; nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100); oncogene Lyt-10; p100; p49/p100; p52; transcription factor NFKB2
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ00653
  • Protein Name
    Nuclear factor NF-kappa-B p100 subunit
  • Scientific Description
    This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Peptide "IEVDLVTHSDPPR" derived from the transcription factor NFKB2 (Nuclear factor NF-kappa-B p100 subunit) conjugated to KLH.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NFkB/NFKB2 p100/p52 using anti-NFkB/NFKB2 p100/p52 antibody (PB9150). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human U87 whole cell lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFkB/NFKB2 p100/p52 antigen affinity purified polyclonal antibody (Catalog # PB9150) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NFkB/NFKB2 p100/p52 at approximately 52 kDa (active form), 120kDa (precursor). The expected band size for NFkB/NFKB2 p100/p52 is at 97 kDa.
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