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Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells.

Anti-NHLRC2 Antibody

HPA038493
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNHLRC2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NHLRC2 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID374354
  • Target name
    NHLRC2
  • Target description
    NHL repeat containing 2
  • Target synonyms
    FINCA, NHL repeat-containing protein 2, 1200003G01Rik, novel NHL repeat domain containing protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NBF2
  • Protein Name
    NHL repeat-containing protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NHLRC2 using anti-NHLRC2 antibody (A17232-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NHLRC2 antigen affinity purified polyclonal antibody (Catalog # A17232-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NHLRC2 at approximately 79 kDa. The expected band size for NHLRC2 is at 79 kDa.
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