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Western blot All lanes: NHLRC2 antibody at 8microg/ml + U251 whole cell lysate Secondary Goat polyclonal to rabbit at 1/10000 dilution Predicted band size: 80, 40 kDa Observed band size: 80 kDa
Western blot All lanes: NHLRC2 antibody at 8microg/ml + U251 whole cell lysate Secondary Goat polyclonal to rabbit at 1/10000 dilution Predicted band size: 80, 40 kDa Observed band size: 80 kDa
Western blot All lanes: NHLRC2 antibody at 8microg/ml + U251 whole cell lysate Secondary Goat polyclonal to rabbit at 1/10000 dilution Predicted band size: 80, 40 kDa Observed band size: 80 kDa

NHLRC2 Antibody

CSB-PA850853LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNHLRC2
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Overview

  • Supplier
    Cusabio
  • Product Name
    NHLRC2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID374354
  • Target name
    NHLRC2
  • Target description
    NHL repeat containing 2
  • Target synonyms
    FINCA, NHL repeat-containing protein 2, 1200003G01Rik, novel NHL repeat domain containing protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8NBF2
  • Protein Name
    NHL repeat-containing protein 2
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NHLRC2 using anti-NHLRC2 antibody (A17232-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NHLRC2 antigen affinity purified polyclonal antibody (Catalog # A17232-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NHLRC2 at approximately 79 kDa. The expected band size for NHLRC2 is at 79 kDa.
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