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Immunohistochemical staining of human tonsil shows moderate cytoplasmic and membranous positivity in germinal center cells.
Immunohistochemical staining of human tonsil shows moderate cytoplasmic and membranous positivity in germinal center cells.
Immunohistochemical staining of human tonsil shows moderate cytoplasmic and membranous positivity in germinal center cells.

Anti-NIN Antibody

HPA005939
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNIN
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NIN
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51199
  • Target name
    NIN
  • Target description
    ninein
  • Target synonyms
    SCKL7, ninein, glycogen synthase kinase 3 beta-interacting protein, hNinein, ninein (GSK3B interacting protein), ninein centrosomal protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8N4C6
  • Protein Name
    Ninein
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Ninein/NIN using anti-Ninein/NIN antibody (A03001-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MOLT4 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ninein/NIN antigen affinity purified polyclonal antibody (Catalog # A03001-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ninein/NIN at approximately 260 kDa. The expected band size for Ninein/NIN is at 243 kDa.
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