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ProductsBack/NIN / Ninein Antibody

NIN / Ninein Antibody

LS-C747816
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetNIN
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NIN / Ninein Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 147kDa/160kDa/236-248kDa, while the observed MW by Western blot was 243kDa.
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 147kDa/160kDa/236-248kDa, while the observed MW by Western blot was 243kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID51199
  • Target name
    NIN
  • Target description
    ninein
  • Target synonyms
    SCKL7, ninein, glycogen synthase kinase 3 beta-interacting protein, hNinein, ninein (GSK3B interacting protein), ninein centrosomal protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Ninein/NIN using anti-Ninein/NIN antibody (A03001-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MOLT4 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Ninein/NIN antigen affinity purified polyclonal antibody (Catalog # A03001-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Ninein/NIN at approximately 260 kDa. The expected band size for Ninein/NIN is at 243 kDa.
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