Anti-NK1.1 [PK-136]

Absolute Antibody

Anti-NK1.1 [PK-136]

9

The antibody reacts with murine NK1.1. NK1.1 is a type II integral membrane glycoprotein with a C-type lectin domain. NK1.1 plays a stimulatory role on natural killer (NK) cells cytotoxicity. Its activation by cross-linking of the receptor induces Ca2+ mo

The antibody was employed for FACS analysis of CD4+8- thymocytes (Arase et al., 1993; PMID: 8419184). The antibody was used for detection of NK1.1 of spleen cells by flow cytometry (Christianson et al., 1996; PMID: 8806787). The antibody was further used for the detection of NK1.1 by flow cytometry analysis (Arase et al., 1992; PMID: 1378629 and Lee et al., 2018; PMID: 30333822 and Coquet et al., 2008; PMID: 18685112). The antibody was employed for in vivo studies, the efficacy of the antibody in depleting the animals of nukappa1.1+ or NK T cells was ascertained by flow cytometric analysis. The reduction was greater than 90% after 1 week of treatment (Nomizo et al., 2005; PMID: 16162272). Finally, the antibody was used for the depletion of NK1.1+ cells in euthymic and thymectomized young C57Bl/6 mice infected with T. cruzi. The depletion of NK1.1 cells caused an increase in the parasitemia in young euthymic mice, but produced no such effects in thymectomized young mice infected with T. cruzi (Tulahuen strain) (Cardillo et al., 2002; PMID: 11841697). The original hybridoma expressed two light chain transcripts, which are both present in the final antibody protein, with only one showing full functionality, with the other light chain potentially mediating off-target binding. This recombinant antibody only features the fully functional light chain, improving antibody performance and on-target specificity.

Flow Cytometry, Other Application

Research Use Only

Monoclonal

PK-136

Klrb1c

killer cell lectin-like receptor subfamily B member 1C

AI462337; CD161; CD161 antigen-like family member C; killer cell lectin-like receptor subfamily A member 1C; killer cell lectin-like receptor subfamily B member 1C; Klrb1b; Ly5; Ly-5; Ly55; Ly55c; ly-55c; Ly59; Ly-59; lymphocyte antigen 55 complex, locus C; lymphocyte antigen 55c; lymphocyte antigen 59; natural killer cell receptor protein NKR-P1C; natural killer cell receptor-P1; natural killer cell surface protein P1-40; natural killer cell-associated antigen 1; Nk; NK-; Nk1; Nk-1; Nk1.; NK-1.; NK1.1; Nk1.2; Nk-1.2; NKR; NKR-; Nkrp; NKRP1; NK-RP1; NKR-P1 40; NKR-P1.9; NKRP140; Nkrp1b; NKR-P1B; Nkrp1c

Mouse

IgG2a

Killer cell lectin-like receptor subfamily B member 1C

This chimeric mouse antibody was made using the variable domain sequences of the original format for improved compatibility with existing reagents assays and techniques.

Mouse

-20°C,2°C to 8°C

12352203