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Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.

Anti-NLRC4 Antibody

HPA006592
Atlas Antibodies
ApplicationsImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetNLRC4
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NLRC4
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID58484
  • Target name
    NLRC4
  • Target description
    NLR family CARD domain containing 4
  • Target synonyms
    AIFEC; CARD, LRR, and NACHT-containing protein; CARD12; caspase recruitment domain family, member 12; caspase recruitment domain-containing protein 12; CED-4-like protein Clan; CLAN; CLAN1; CLANA; CLANB; CLANC; CLAND; CLR2.1; FCAS4; ICE-protease activating factor; IPAF; NLR family CARD domain-containing protein 4; NOD-like receptor C4; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NPP4
  • Protein Name
    NLR family CARD domain-containing protein 4
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CARD12 using anti-CARD12 antibody (PB9673). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: 22RV1 Whole Cell Lysate, Lane 2: RH35 Whole Cell Lysate, Lane 3: NIH3T3 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARD12 antigen affinity purified polyclonal antibody (Catalog # PB9673) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CARD12 at approximately 116 kDa. The expected band size for CARD12 is at 116 kDa.
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