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Immunohistochemistry of paraffin-embedded human colon tissue using CSB-PA873615ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon tissue using CSB-PA873615ESR1HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human colon tissue using CSB-PA873615ESR1HU at dilution of 1:100

NLRC4 Antibody

CSB-PA873615ESR1HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNLRC4
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Overview

  • Supplier
    Cusabio
  • Product Name
    NLRC4 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID58484
  • Target name
    NLRC4
  • Target description
    NLR family CARD domain containing 4
  • Target synonyms
    AIFEC, CARD12, CLAN, CLAN1, CLANA, CLANB, CLANC, CLAND, CLR2.1, FCAS4, IPAF, NLR family CARD domain-containing protein 4, CARD, LRR, and NACHT-containing protein, CED-4-like protein Clan, ICE-protease activating factor, NOD-like receptor C4, caspase recruitment domain family, member 12, caspase recruitment domain-containing protein 12, nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NPP4
  • Protein Name
    NLR family CARD domain-containing protein 4
  • Scientific Description
    Key component of inflammasomes that indirectly senses specific proteins from pathogenic bacteria and fungi and responds by assembling an inflammasome complex that promotes caspase-1 activation, cytokine production and macrophage pyroptosis.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CARD12 using anti-CARD12 antibody (PB9673). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: 22RV1 Whole Cell Lysate, Lane 2: RH35 Whole Cell Lysate, Lane 3: NIH3T3 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARD12 antigen affinity purified polyclonal antibody (Catalog # PB9673) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CARD12 at approximately 116 kDa. The expected band size for CARD12 is at 116 kDa.
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