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Immunohistochemical staining of human testis shows strong cytoplasmic positivity in Leydig cells.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in Leydig cells.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in Leydig cells.

Anti-NLRP9 Antibody

HPA042623
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNLRP9
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NLRP9 Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID338321
  • Target name
    NLRP9
  • Target description
    NLR family pyrin domain containing 9
  • Target synonyms
    CLR19.1, NALP9, NOD6, PAN12, NACHT, LRR and PYD domains-containing protein 9, NACHT, LRR and PYD containing protein 9, NACHT, leucine rich repeat and PYD containing 9, PYRIN and NACHT-containing protein 12, nucleotide-binding oligomerization domain protein 6, nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7RTR0
  • Protein Name
    NACHT, LRR and PYD domains-containing protein 9
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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MSDS

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Figure 1. Western blot analysis of NLRP9 using anti-NLRP9 antibody (A14119-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRP9 antigen affinity purified polyclonal antibody (Catalog # A14119-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NLRP9 at approximately 113 kDa. The expected band size for NLRP9 is at 113 kDa.
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