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The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA447017(NLRP9 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA447017(NLRP9 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using CSB-PA447017(NLRP9 Antibody) at dilution 1/40, on the right is treated with synthetic peptide. (Original magnification: x200)

NLRP9 Antibody

CSB-PA447017
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNLRP9
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Overview

  • Supplier
    Cusabio
  • Product Name
    NLRP9 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID338321
  • Target name
    NLRP9
  • Target description
    NLR family pyrin domain containing 9
  • Target synonyms
    CLR19.1, NALP9, NOD6, PAN12, NACHT, LRR and PYD domains-containing protein 9, NACHT, LRR and PYD containing protein 9, NACHT, leucine rich repeat and PYD containing 9, PYRIN and NACHT-containing protein 12, nucleotide-binding oligomerization domain protein 6, nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ7RTR0
  • Protein Name
    NACHT, LRR and PYD domains-containing protein 9
  • Scientific Description
    The protein encoded by this gene belongs to the NALP protein family. Members of the NALP protein family typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminal leucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD). This protein may play a regulatory role in the innate immune system as similar family members belong to the signal-induced multiprotein complex, the inflammasome, that activates the pro-inflammatory caspases, caspase-1 and caspase-5.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NLRP9 using anti-NLRP9 antibody (A14119-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NLRP9 antigen affinity purified polyclonal antibody (Catalog # A14119-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NLRP9 at approximately 113 kDa. The expected band size for NLRP9 is at 113 kDa.
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