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Immunohistochemical staining of human bronchus shows strong cytoplasmic positivity in endothelial cells and respiratory epithelial cells.
Immunohistochemical staining of human bronchus shows strong cytoplasmic positivity in endothelial cells and respiratory epithelial cells.
Immunohistochemical staining of human bronchus shows strong cytoplasmic positivity in endothelial cells and respiratory epithelial cells.

Anti-NME6 Antibody

HPA017909
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetNME6
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-NME6
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10201
  • Target name
    NME6
  • Target description
    NME/NM23 nucleoside diphosphate kinase 6
  • Target synonyms
    inhibitor of p53-induced apoptosis-alpha; IPIA-ALPHA; NDK 6; NDP kinase 6; NM23-H6; non-metastatic cells 6, protein expressed in (nucleoside-diphosphate kinase); nucleoside diphosphate kinase 6
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75414
  • Protein Name
    Nucleoside diphosphate kinase 6
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NME6 using anti-NME6 antibody (A10922-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Lncap whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NME6 antigen affinity purified polyclonal antibody (Catalog # A10922-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NME6 at approximately 21 kDa. The expected band size for NME6 is at 21 kDa.
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